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Key Documents

MAB3326

Sigma-Aldrich

Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12

clone IV-4H12, Chemicon®, from mouse

Synonym(s):

Anti-BSVD, Anti-COL4A1s, Anti-PADMAL, Anti-RATOR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

IV-4H12, monoclonal

species reactivity

human

should not react with

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL4A1(1282)

Specificity

Specifically reactive with human Type IV Collagen.

Immunogen

Epitope: 7S Domain

Application

Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12 is validated for use in ELISA, WB, IH(P) for the detection of Collagen Type IV.
Western blot. Detects human type IV collagen from human placental tissues at 2 μg/mL, giving a band at approximately 92 kDa. Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine serum albumin.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Analysis Note

Control
POSITIVE CONTROL: human placental tissues.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Manufactured by Daiichi Fine Chemical Co., Ltd

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rashmi Ramakrishnan et al.
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Degeneration of the primary motor cortex is a defining feature of amyotrophic lateral sclerosis (ALS), which is associated with the accumulation of microscopic protein aggregates in neurons and glia. However, little is known about the quantitative burden and pattern of
Yuliya Nashchekina et al.
Polymers, 14(21) (2022-11-12)
The lack of suitable functional groups for cell adhesion on the surface of Polycaprolactone (PCL) is one of the main limitations in order to use PCL for biomedical applications. The aim of this research is to modify the PCL film
Tiina A Jokela et al.
Frontiers in cell and developmental biology, 6, 41-41 (2018-05-03)
The existence of rare cancer cells that sporadically acquire drug-tolerance through epigenetic mechanisms is proposed as one mechanism that drives cancer therapy failure. Here we provide evidence that specific microenvironments impose non-sporadic expression of proteins related to epithelial plasticity and

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