1.07232

BPLS agar

for the isolation of Salmonella

• Synonym(s): Brilliant-green phenol-red lactose sucrose agar, Brillant-green phenol-red lactose sucrose agar
• UNSPSC Code: 41106213

Properties

• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• manufacturer/tradename: GranuCult^®
• technique(s): microbiological culture: suitable
• pH: 6.8-7.0 (37 °C, 52 g/L in H₂O, after autoclaving)
• solubility: 52 g/L
• bulk density: 600 kg/m³
• application(s): clinical; microbiology
• storage temp.: 15-25°C
• suitability: Salmonella spp.

Description

General description

for the isolation of Salmonella

Analysis Note

Appearance (clearness)clearAppearance (colour)dark redSolidification point32 - 36 °CGel strength (Gelomat assay)≥ 50 gpH-value (25 °C)6.7 - 7.1Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))10 - 1000Inoculum on reference medium (Salmonella choleraesuis ATCC 13312)10 - 1000Inoculum on reference medium (Salmonella enteritidis NCTC 5188 (WDCM 00119))10 - 1000Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))10 - 1000Inoculum on reference medium (Proteus hauseri ATCC 13315)10 - 1000Inoculum (Staphylococcus aureus ATCC 25923 (WDCM 00034))10⁴Inoculum (Enterococcus faecalis ATCC33186)10⁴Inoculum (Bacillus subtilis ATCC 6633 (WDCM 00003))10⁴Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031))Colony count (Salmonella choleraesuis ATCC 13312)Colony count (Salmonella enteritidis NCTC 5188 (WDCM 00119))Colony count (Escherichia coli ATCC 25922 (WDCM 00013))Colony count (Proteus hauseri ATCC 13315)Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))≥ 40%Recovery on test medium (Salmonella choleraesuis ATCC 13312)≥ 40%Recovery on test medium (Salmonella enteritidis NCTC 5188 (WDCM 00119))≥ 40%Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))no LimitRecovery on test medium (Proteus hauseri ATCC 13315)no limitGrowth (Bacillus subtilis ATCC 6633 (WDCM 00003))no limitGrowth (Enterococcus faecalis ATCC 33186)no limitGrowth (Staphylococcus aureus ATCC 25923 (WDCM 00034))no limitColony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031))pinkColony colour (Salmonella choleraesuis ATCC 13312)pinkColony colour (Salmonella enteritidis NCTC 5188 (WDCM 00119))pinkColony colour (Escherichia coli ATCC 25922 (WDCM 00013))yellowColony colour (Proteus hauseri ATCC 13315)yellowIncubation: 24 hrs.; 35 °C; aerobic.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3