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G005382

HPLC Analysis of Aldehydes and Ketones as DNPH Derivatives on Ascentis® Express C18

application for air monitoring, application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)
mobile phase: [A] water; [B] acetonitrile; (40:60, A:B)
flow rate: 2 mL/min
pressure: 4685 psi (323 bar)
column temp.: 30 °C
detector: UV, 360 nm
injection: 5 μL
sample: Sample in water

suitability

application for HPLC
application for air monitoring

application(s)

air monitoring
industrial hygiene

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the separation of aldehyde/ketone-DNPH derivatives.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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