T7024

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, for molecular biology, ≥99% (GC)

• Synonym(s): 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA
• Linear Formula: (CH₃)₂NCH₂CH₂N(CH₃)₂
• CAS Number: 110-18-9
• Molecular Weight: 116.20
• Beilstein: 1732991
• EC Number: 203-744-6
• MDL number: MFCD00008335
• UNSPSC Code: 41105319
• PubChem Substance ID: 24900242
• NACRES: NA.31

Properties

• grade: for molecular biology
• product line: BioReagent
• Assay: ≥99% (GC)
• expl. lim.: 9.08 %
• refractive index: n20/D 1.4179 (lit.)
• bp: 120-122 °C (lit.)
• mp: −55 °C (lit.)
• density: 0.775 g/mL at 20 °C (lit.)
• foreign activity: DNase, RNase, protease, none detected
• SMILES string: CN(C)CCN(C)C
• InChI: 1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3
• InChI key: KWYHDKDOAIKMQN-UHFFFAOYSA-N

Description

Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Quantity

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Safety Information

• Pictograms: GHS02,GHS06,GHS05
• Signal Word: Danger
• Hazard Statements: H225,H302,H314,H331
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 61.7 °F - closed cup
• Flash Point(C): 16.5 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves