Skip to Content
Merck
All Photos(1)

Documents

RAB0904

Sigma-Aldrich

Rat Ins1 / Insulin ELISA Kit

Synonym(s):

Insulin

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 μIU/mL
standard curve range: 4.69–300 μIU/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Ins1(24505)

General description

The antibody pair provided in this kit recognizes Rat Insulin.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Rat Ins1 / Insulin ELISA Kit has been used to measure serum insulin concentration.

Biochem/physiol Actions

Certain organisms such as mice, rat and xenopus possess two non-allelic insulin genes. These genes are Ins1 and Ins2, which are both functional. They encode insulin that is produced in a 1:2 ratio, respectively in the pancreas. The two genes are differentially expressed in mouse. The Ins1 gene maps to mouse chromosome 19 in the telomeric region. The insulin that is synthesized in the pancreas is crucial in maintaining glucose homeostasis. Loss of Ins-1 and Ins-2 expression leads to insulin deficient mice with diabetes mellitus, ketoacidosis, liver steatosis and retarded growth.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Jingjing Li et al.
Neural plasticity, 2019, 7920540-7920540 (2019-03-27)
We investigated the effects of aerobic exercise on the expression of hippocampal synaptic plasticity-associated proteins in rats with type 2 diabetes and their possible mechanisms. A type 2 diabetes rat model was established with 8 weeks of high-fat diet combined
Hongtao Tan et al.
Molecular medicine (Cambridge, Mass.), 28(1), 58-58 (2022-05-21)
Glabridin (Glab) is a bioactive component of licorice that can ameliorate diabetes, but its role in diabetic nephropathy (DN) has seldom been reported. Herein, we explored the effect and underlying mechanism of Glab on DN. The bioactive component-target network of
Sadi Loai et al.
Frontiers in cardiovascular medicine, 8, 715400-715400 (2021-08-07)
Aim: To perform a deep cardiac phenotyping of type II diabetes in a rat model, with the goal of gaining new insight into the temporality of microvascular dysfunction, cardiac dysfunction, and exercise intolerance at different stages of diabetes. Methods and
Amanda C Okolie et al.
Bioscience reports, 39(2) (2017-08-10)
Recent studies have shown that Type 2 diabetes (T2D) in rats can result through a synergy that links obesity to insulin resistance and β-cell dysfunction. The present study achieved T2D via high fructose (20%w/v, p.o.), streptozotocin single dose (40 mg/kg
Tian Shen et al.
Experimental and therapeutic medicine, 16(4), 3121-3128 (2018-09-15)
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease. It is asymptomatic at presentation and is frequently identified among individuals with metabolic dysfunction, including obesity and diabetes. NAFLD is primarily characterized by the accumulation of triacylglycerol in the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service