O1391

Oil Red O solution

0.5% in isopropanol

• Synonym(s): Solvent Red 27, Sudan Red 5B
• Empirical Formula (Hill Notation): C₂₆H₂₄N₄O
• CAS Number: 1320-06-5
• Molecular Weight: 408.49
• EC Number: 200-661-7
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• form: liquid
• Quality Level: 200
• concentration: 0.5% in isopropanol
• color: dark red
• εmax: 0.20-0.50 at 510-517 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• InChI: 1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
• InChI key: HJAYEODIXUYVIC-LTFAAIOASA-N

Description

General description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H319,H336
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 53.6 °F - closed cup
• Flash Point(C): 12.0 °C - closed cup