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F3512

Sigma-Aldrich

Anti-Human IgG (whole molecule)−FITC antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Goat Anti-Human IgG (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunofluorescence: 1:32-1:64 using Hep2 cells

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Specificity of anti-human IgG (whole molecule)-FITC antibody for human IgG is determined by immunoelectrophoresis against purified human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. The two heavy chains and two light chains of IgG are connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Immunogen

Pooled normal human serum

Application

Anti-Human IgG (whole molecule)-FITC antibody has been used:
  • in nucleolar staining
  • in single color fluorescent labeling for the detection of lytically induced cells
  • to detect the model antigen
  • in immunohistochemistry
  • in immunofluorescence

Anti-Human IgG (whole molecule)-FITC antibody may be used for anti-nuclear antibody (ANA) assays. The antibody may also be used in FACS-based assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Cheng et al.
Molecular and cellular biology, 21(18), 6198-6209 (2001-08-18)
Sphingolipids are major components of the plasma membrane of eukaryotic cells and were once thought of merely as structural components of the membrane. We have investigated effects of inhibiting sphingolipid biosynthesis, both in germinating spores and growing hyphae of Aspergillus
A model system for concurrent detection of antigen and antibody based on immunological fluorescent method
Cao Y C
Journal of Spectroscopy (New York, NY, United States), 2015 (2015)
Rhiannon S McBean et al.
Transfusion, 55(6 Pt 2), 1451-1456 (2014-12-20)
Until recently, SARAH (SARA) was a low-frequency antigen within the 700 series (700.052). SARA was discovered in Australia and subsequently described in Canada where anti-SARA was implicated in severe hemolytic disease of the fetus and newborn (HDFN). This study investigated
Ontogeny and distribution of Fc gamma receptors in the human placenta. Transport or immune surveillance?
Bright N A, et al.
Journal of Anatomy, 184(Pt 2), 297-297 (1994)
Dung Nguyen et al.
Viruses, 13(5) (2021-05-01)
As of April 2021, the COVID-19 pandemic has swept through 213 countries and infected more than 132 million individuals globally, posing an unprecedented threat to human health. There are currently no specific antiviral treatments for COVID-19 and vaccination programmes, whilst

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