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CL6B200

Sigma-Aldrich

Sepharose™ CL-6B Size Exclusion Resin

Cross-linked, 90-350 mesh

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About This Item

CAS Number:
62610-50-8
MDL number:
MFCD00148280
UNSPSC Code:
23151817
NACRES:
NA.84
Pricing and availability is not currently available.

Product Name

Sepharose™ CL-6B, Cross-linked

Quality Level

200

form

suspension

bead diameter

40-165 μm

pore size

10,000-1,000,000 fractionation range (Dextrans)
10,000-4,000,000 fractionation range (Globular proteins)

storage temp.

2-8°C

Related Categories

General description

Sepharose CL is a cross-linked derivative of sepharose, prepared by reacting sepharose with 2,3-dibromopropanol under strongly alkaline conditions.[1]

Application

Sepharose CL-6B is used in affinity chromatography, protein chromatography, gel filtration chromatography, separation media and resins. Sepharose™ CL-6B has been used to study in vitro inhibition of human melanoma cells and to gather data in other valuable anticancer studies.
Sepharose™CL-6B has been used for the purification of glucoamylase enzyme[2] and targeted ribonucleoprotein (RNP)[3].

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H225

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW0713
MKCW1843
MKCT8362
MKCT4884
MKCS9766

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M Schweizer et al.
Molecular & general genetics : MGG, 203(3), 479-486 (1986-06-01)
FAS1, the structural gene of the pentafunctional fatty acid synthetase subunit beta in Saccharomyces cerevisiae has been sequenced. Its reading frame represents an intron-free nucleotide sequence of 5,535 base pairs, corresponding to a protein of 1,845 amino acids with a
W Chen et al.
Journal of bacteriology, 175(17), 5677-5682 (1993-09-01)
A membrane-associated ATPase with an M(r) of approximately 510,000 and containing subunits with M(r)s of 80,000 (alpha), 55,000 (beta), and 25,000 (gamma) was isolated from the methanogen Methanococcus voltae. Enzymatic activity was not affected by vanadate or azide, inhibitors of
Ru-Ping Zhang et al.
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 30(6), 561-564 (2002-08-09)
Luffin B, a plan single-chain ribosome inactivating protein, was purified from seeds of Luffa cylindrica by Blue Sepharose CL-6B affinity chromatography. An immunotoxin was constructed with luffin B and Ng76, a monoclonal antibody to human melanoma cell M(21). Luffin B-Ng76
C C Chadwick et al.
Archives of biochemistry and biophysics, 252(2), 348-356 (1987-02-01)
As isolated by our recently developed large-scale procedure, the Neurospora plasma membrane H+-ATPase exists as a homogeneous, oligomeric complex of 105,000-Da monomers with a molecular mass equivalent to a spherical protein of about 1 million Da, as judged by its
Amylolytic fungi in starter cakes for rice beer production
Das AJ, et al.
The Journal of General and Applied Microbiology, 63(4), 236-245 (2017)
View All Related Papers

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. セファロース™CL-6B(CAS 62610-50-8)はビーズには何も担持されていない無担持のものでしょうか？

    1 answer

    1. This is a product of Cytiva. The manufacturer notes that this matrix is a well-proven cross-linked agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. The matrix contains 6% cross-linked agarose size exclusion chromatography resins. but there is no added functionality or support.

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