MAB1864
Anti-Tubulin Antibody, clone YL1/2
clone YL1/2, Chemicon®, from rat
Synonym(s):
Anti-B-ALPHA-1, Anti-LIS3, Anti-TUBA3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
YL1/2, monoclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... TUBA1A(7846)
General description
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
Specificity
MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
Immunogen
Epitope: The epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Yeast tubulin
Application
Detect Tubulin using this Anti-Tubulin Antibody, clone YL1/2 validated for use in ELISA, IH, IP, RIA & WB.
Immunohistochemistry:
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Quality
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
Target description
55 kDa
Linkage
Replaces: 04-1117
Physical form
Format: Purified
Protein A purified
Purified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide.
Storage and Stability
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
All tissue
All tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Peter T Ruane et al.
Scientific reports, 6, 27456-27456 (2016-06-09)
Microtubules and their associated proteins (MAPs) underpin the polarity of specialised cells. Adenomatous polyposis coli (APC) is one such MAP with a multifunctional agenda that requires precise intracellular localisations. Although APC has been found to associate with kinesin-2 subfamily members
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Lee, S; Liu, HP; Lin, WY; Guo, H; Lu, B
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Cruz-Monserrate, Z; O'Connor, KL
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Williams, LS; Ganguly, S; Loiseau, P; Ng, BF; Palacios, IM
Development null
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