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516620

Sigma-Aldrich

Peroxynitrite

liquid, biological oxidizing agent, Calbiochem®

Synonym(s):

Nitroxyl anion, Peroxynitrous acid

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.77

product name

Peroxynitrite, Synthesized from isoamyl nitrite and hydrogen peroxide.

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

color

yellow

shipped in

wet ice

storage temp.

−70°C

General description

Note: 1 set = 2 x 1 ml.
Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO–) is a strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. It has been implicated in a variety of patho-physiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock. This product decomposes or isomerizes at pH 8 or less; the half-life at pH 7.5 is ~1.5 s. ε302 nm = 1670M-1cm-1 in 0.1 MNaOH.
Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO) is a cell-permeable strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. Has been implicated in a variety of pathophysiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Oxidizer (K)

Physical form

Supplied in 4.7% NaOH at 160-200 mM.

Preparation Note

Peroxynitrite is soluble in aqueous buffers that have been pre-purged with an inert gas and pre-cooled to 4°C.

Reconstitution

Protect from heat, light, metal ions, and air. Peroxynitrite is unstable; thaw rapidly, dispense into aliquots, purge tubes with an inert gas, and freeze -70°C. Activity decreases ~2% /day at -20°C.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Marla, S.S., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14243.
Sakuma, S., et al. 1997. Biochem. Biophys. Res. Commun. 230, 476.
Lymar, S.V., et al. 1996. Biochemistry 35, 7855.
Uppu, R.M., and Pryor, W.A. 1996. Methods Enzymol. 269, 322.
Ischiropoulos, H., and al-Medhi, A.B. 1995. FEBS Lett. 364, 279.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Satoshi Fukuda et al.
Shock (Augusta, Ga.), 52(5), e92-e99 (2018-12-01)
Vascular hypo-responsiveness to vasopressors during septic shock is a challenging problem. This study is to test the hypothesis that reactive nitrogen species (RNS), such as peroxynitrite, are major contributing factors to vascular hypo-responsiveness in septic shock. We hypothesized that adjunct
Yash Mantri et al.
Nanoscale, 12(19), 10511-10520 (2020-05-13)
Accumulation of reactive oxygen and nitrogen species (RONS) can induce cell damage and even cell death. RONS are short-lived species, which makes direct, precise, and real-time measurement difficult. Biologically-relevant RONS levels are in the nM-μM scale; hence, there is a

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