14-404-M
ZAP-70 Protein, active, 20 µg
Active, recombinant, human, full length ZAP-70 containing a C-terminal His6 tag, for use in Kinase Assays.
Synonym(s):
70 kDa zeta-chain associated protein, SRK, Syk-related tyrosine kinase
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32
biological source
human
Quality Level
recombinant
expressed in Sf21 cells
form
liquid
shelf life
6 mo.
mol wt
Mw 70 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... ZAP70(7535)
General description
Research area: CELL SIGNALING
Product Source: expressed in Sf21 Recombinant, human, full length ZAP-70 containing a C-terminal His6-tag
Zeta-chain-associated protein kinase 70 (ZAP-70) is a cytoplasmic tyrosine kinase that consists of tandem SH2 (tSH2) domains at the beginning, linked with a long linker region, and a tyrosine kinase domain at the C-terminal end.
Product Source: expressed in Sf21 Recombinant, human, full length ZAP-70 containing a C-terminal His6-tag
Zeta-chain-associated protein kinase 70 (ZAP-70) is a cytoplasmic tyrosine kinase that consists of tandem SH2 (tSH2) domains at the beginning, linked with a long linker region, and a tyrosine kinase domain at the C-terminal end.
Biochem/physiol Actions
Protein Target: ZAP-70
Target Sub-Family: TK
Zeta-chain-associated protein kinase 70 (ZAP-70) plays a vital role in the development and function of T cells and natural killer cells. It interacts directly with the activated T-cell receptor and regulates downstream signals. In addition, ZAP-70 also mediates B-cell receptor (BCR) signaling. Mutations in ZAP-70 have been associated with severe combined immunodeficiency (SCID), chronic lymphocytic leukemia (CLL) , and the development of autoreactive T cells that attack the joints.
Target Sub-Family: TK
Zeta-chain-associated protein kinase 70 (ZAP-70) plays a vital role in the development and function of T cells and natural killer cells. It interacts directly with the activated T-cell receptor and regulates downstream signals. In addition, ZAP-70 also mediates B-cell receptor (BCR) signaling. Mutations in ZAP-70 have been associated with severe combined immunodeficiency (SCID), chronic lymphocytic leukemia (CLL) , and the development of autoreactive T cells that attack the joints.
Quality
routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1) substrate peptide
Physical form
Ni2+/NTA-agarose
Storage and Stability
6 months at -20C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
ZAP-70: an essential kinase in T-cell signaling
Wang H, et al.
Cold Spring Harbor Perspectives in Biology, 2(5), a002279-a002279 (2010)
Experimental Models for Rheumatoid Arthritis
Rikard Holmdahl
Journal of Separation Science, 400-412 (2013)
ZAP-70 in Signaling, Biology, and Disease
Au-Yeung BB, et al.
Annual Review of Immunology, 36, 127-156 (2018)
The Structural Basis for Activation and Inhibition of ZAP-70 Kinase Domain
Huber RG, et al.
PLoS Computational Biology, 11(10), e1004560-e1004560 (2015)
D H Chu et al.
Immunological reviews, 165, 167-180 (1998-12-16)
The processes of T-cell development and activation employ similar immature and mature receptors as well as similar signal transduction pathways to achieve different outcomes. Many signaling molecules are shared between the receptor signaling pathways, including two families of cytoplasmic protein
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