G005833

HPLC Analysis of Spice Cannabinoids JWH-018, JWH-073 and Metabolites on Ascentis^® Express C18

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U); mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in water:acetonitrile (10:90); gradient: 20% B held for 3 min; 20 to 100% B in 0.1 min; 100% B held for 2 min; flow rate: 0.5 mL/min; pressure: 2150 psi (148 bar); column temp.: 35 °C; detector: UV, 315 nm; injection: 2 μL; sample: 17 μg/mL each in acetonitrile
• suitability: application for HPLC
• application(s): forensics and toxicology

Description

Analysis Note

Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. A rapid separation of six of these compounds on an Ascentis Express C18 column is shown here. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

JWH-073 4-Butanoic acid metabolite solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
JWH-018 5-Pentanoic acid metabolite solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
JWH-073 solution, 100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®
JWH-018 solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®