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G005515

HPLC Analysis of Thyroxine Enantiomers on Astec® CHIROBIOTIC® TAG

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)
mobile phase: 1% ammonium formate in methanol
flow rate: 1 mL/min
pressure: 826 psi (57 bar)
column temp.: 25 °C
detector: UV, 220 nm
injection: 10 μL
sample: each enantiomer 0.1 mg/L in methanol

suitability

application for HPLC

application(s)

clinical
life science and biopharma

Analysis Note

Baseline resolution of the chiral enantiomers of DL-Thyroxine is achieved by polar ionic mode on the Astec CHIROBIOTIC TAG with the use of an LC/MS-compatible mobile phase.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    L-Thyroxine, ≥98% (HPLC)
    D-Thyroxine, 99%

analytical column

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standard

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