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G005392

HPLC Analysis of Diet Soda Additives on Ascentis® Express C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 μm particles (53818-U)
mobile phase: 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
pressure: 3191 psi (220 bar)
flow rate: 3 mL/min
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: (Diet Soda 100 - 500 μg/mL in buffer)

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet cola.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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