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G001488A

HPLC Analysis of Lysozyme, Myoglobin and Impurities on Discovery® BIO Wide Pore C5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO Wide Pore C5, 15cm × 4.6mm I.D., 5μm particles (568422-U)
mobile phase: [A] (75:25) 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (25:75) 0.1% TFA in water:0.1% TFA in acetonitrile
gradient: 0-100% B in 25 min
flow rate: 1.0 mL/min
column temp.: ambient
detector: UV, 220 nm
injection: 12 μL in 0.1% TFA

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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