Skip to Content
Merck
All Photos(1)

Documents

82021-U

Hamilton® PRP-1 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.1 mm, stainless steel hardware

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Hamilton PRP-1 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.1 mm, stainless steel hardware

material

stainless steel column

Agency

suitable for USP L21

manufacturer/tradename

Hamilton

packaging

1 ea of

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.1 mm

matrix

PS-DVB particle platform
polymeric

matrix active group

polymer

particle size

5 μm

pore size

100 Å

operating pH

1-14

separation technique

reversed phase

Looking for similar products? Visit Product Comparison Guide

General description

PRP-1 is a co-polymer of poly (styrene-divinylbenzene). Its surface is chemically neither homogenous nor stable during use. Hamilton PRP-1 HPLC Column, is used for large scale purification of oligonucleotides, generally used in NMR studies.
The Hamilton PRP-1, polymeric reversed phase columns are Ideal for high pH (pH 8-13) operation or with analytes that give poor chromatogrpahy on silica-based columns.

Application

Hamilton PRP-1 HPLC Column was used in experimental studies employing liquid chromatography (LC); with reversed phase separation (RPLC) using only pressurized hot water (PHW) as a mobile phase. It also is suitable for being used in large-scale purification of synthetic oligonucleotides, with a phosphate/methanol/acetonitrile solvent system.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Large-scale purification of synthetic oligonucleotides and carcinogen-modified oligodeoxynucleotides on a reverse-phase polystyrene (PRP-1) column.
Guanjin H and Krugh TR.
Analytical Biochemistry, 190 (1), 21-25 (1990)
Liquid chromatography at elevated temperatures with pure water as the mobile phase.
Kari H and Riekkola ML.
Trends in Analytical Chemistry, 27 (1), 1-14 (2008)
Characterization of some commercial poly (styrene-divinylbenzene) copolymers for reversed-phase HPLC1.
Stuurman HW
Chromatographia, 23 (5), 341-349 (1987)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service