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Supelco

Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 4.6 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L43

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 4.6 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application



  • Research on the Synthesis of Zinc-Ammonium Phosphate Using Galvanic Waste Sludge as a Source of Zinc.: This research investigates the synthesis of zinc-ammonium phosphate utilizing galvanic waste sludge, highlighting the analytical methods involving hydrochloric acid in the chemical recycling process (Morgovan et al., 2024).


  • Chile′s agricultural research institute plant tissue analysis dataset.: This dataset encompasses analytical methods used for plant tissue analysis, with hydrochloric acid playing a key role in the preparation and analysis processes (Corradini et al., 2023).


  • Determination of three plant growth regulators in Dendrobium officinale and Anoectochilus roxburghii by three-phase hollow fiber liquid phase microextraction- high performance liquid chromatography.: The study details a method using hydrochloric acid for the extraction and analysis of plant growth regulators, enhancing the accuracy of high-performance liquid chromatography techniques (Wu et al., 2023).




Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

This application note describes the LC-MS-based quantitative analysis of known nitrosamine impurities following procedures 1 and 3 as given in USP general chapter <1469>.

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Protocols

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

Related Content

Accurately detect and quantify trace nitrosamines (NDMA, NDEA, NEIPA, NDIPA, NDBA, etc.) in pharmaceutical drugs using our complete product portfolio and application guides for LC-MS and GC-MS. Order high-quality reference standards, columns, filters & more.

Chromatograms

application for HPLCapplication for HPLCapplication for HPLC

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