T6454

Anti-Timeless (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-TIM, Anti-TIM1, Anti-Timeless1, Anti-hTIM
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~160 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using HEK-293T cells over expressing human Timeless; indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human Timeless; western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing human Timeless
• UniProt accession no.: Q9UNS1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TIMELESS(8914)

Description

General description

The gene TIMELESS is located on the human chromosome at 12q13.3. The encoded protein is a component of the replisome complex.

Specificity

Anti-Timeless (C-terminal) recognizes human Timeless (also known as hTIM, TIM, TIM1, Timelesss1).

Application

Anti-Timeless (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Timeless (Tim) forms a complex with Tim interacting protein (Tipin), found by two-hybrid screening. It associates with components of the replication fork complex through direct binding with the (RPA) and are important for efficient DNA synthesis. Timeless plays a role in replication checkpoint responses to genotoxic stress. It also plays an important role in the maintenance of genome stability during unperturbed DNA replication. Overexpression of the TIMELESS gene is associated with cervical cancer. Annihilation of this gene induces apoptosis and cell senescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves