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SRP0357

Sigma-Aldrich

NEK2 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥65% (SDS-PAGE)

Synonym(s):

HsPK21, NEK2A, NIMA (never in mitosis gene a)-related kinase 2, NLK1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥65% (SDS-PAGE)

form

aqueous solution

mol wt

53 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

1.2 mg/mL

NCBI accession no.

NM_002497

UniProt accession no.

P51955

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NEK2(4751)

General description

Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal HIS6-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lynda Fletcher et al.
Biochimica et biophysica acta, 1744(2), 89-92 (2005-06-14)
Two splice variants of Nek2 kinase, a member of the NIMA-related family, have been identified as Nek2A and Nek2B. Nek2A regulates centrosome disjunction, spindle formation checkpoint signaling, and faithful chromosome segregation. A specific role for Nek2B has not yet been
Margarida Fardilha et al.
Annals of the New York Academy of Sciences, 1030, 468-478 (2005-01-22)
Mammalian sperm were previously shown to express the PP1gamma2 isoform of protein phosphatase 1 (PP1) as well as its regulatory proteins inhibitor 2 and glycogen synthase kinase 3. Furthermore, the development of sperm motility during transit through the epididymis correlates

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