SRP0168
EED human
recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
Synonym(s):
HEED, Rinchik 5, WAIT1, WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
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biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
51 kDa
packaging
pkg of 50 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EED(8726)
General description
EED (ectoderm development protein) is the part of the polycomb repressive complex 2 (PRC2) where it interacts with EZH2 (enhancer of zeste homolog 2). The protein has seven WD domains (β-transducin domains). The EED gene is mapped to human chromosome 11q14.
Human Embryonic Ectoderm Development (hEED), also known as WAIT-1, GenBank Accession No. NM_003797, amino acids 2-end with N-terminal FLAG-tag, MW = 51kDa, expressed in Sf9 cells via a baculovirus expression system.
Human Embryonic Ectoderm Development (hEED), also known as WAIT-1, GenBank Accession No. NM_003797, amino acids 2-end with N-terminal FLAG-tag, MW = 51kDa, expressed in Sf9 cells via a baculovirus expression system.
Biochem/physiol Actions
EED (ectoderm development protein) is required for proper EZH2 (enhancer of zeste homolog 2)-mediated methyltransferase activity. It also associates with PRC1 (polycomb repressive complex 1) and helps in bringing PRC1 to H3K27me3 (trimethylated lysine residue at position 27 in the protein histone H3) loci. EED also increases PRC1-mediated H2A (histone 2A) ubiquitin E3 ligase activity. Mutations in the EED gene are associated with overgrowth, intellectual disability and dysmorphic features. Change in the activity of EED is linked with the pathogenesis of myeloid disorders.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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EED mutants impair polycomb repressive complex 2 in myelodysplastic syndrome and related neoplasms.
Ueda T, et al.
Leukemia, 26, 2557-2557 (2012)
EED-associated overgrowth in a second male patient.
Cohen AS and Gibson WT
Journal of Human Genetics, 61, 831-831 (2016)
The central role of EED in the orchestration of polycomb group complexes.
Cao Q, et al.na
Nature Communications, 5, 3127-3127 (2014)
Ioannis Papoulidis et al.
Molecular cytogenetics, 8, 71-71 (2015-09-22)
Interstitial deletions of the long arm of chromosome 11 are rare, and they could be assumed as non-recurrent chromosomal rearrangements due to high variability of the size and the breakpoints of the deleted region. The exact region of the deletion
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