SAB4702055

Monoclonal Anti-CD63 Biotin produced in mouse

• Synonym(s): Granulophysin, LAMP-3, ME491, MLA1, OMA81H, TSPAN30, Tetraspanin-30, Tspan-30
• UNSPSC Code: 12352203

Properties

• conjugate: biotin conjugate
• Quality Level: 100
• antibody product type: primary antibodies
• clone: MEM-259, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 1 mg/ml
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1
• UniProt accession no.: P08962
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD63(967)

Description

General description

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

Specificity

The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Immunogen

HPB-ALL T cell line

Physical form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Storage and Stability

Store at 2-8°C. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable