immunoblotting: 0.25-0.5 μg/mL using human recombinant peptide of MR-Pro ADM (amino acid 45-92). indirect ELISA: 30-60 ng/mL using human recombinant peptide of MR-Pro ADM (amino acid 45-92) for coating.
Monoclonal Anti-MR-Pro ADM (mid-regional pro adrenomedullin) (mouse IgG1 isotype) is derived from the hybridoma 2E12E6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human recombinant protein. Mid-regional pro-adrenomedullin (MR-proADM) is obtained as a by-product of post-translational processing of prepro adrenomedullin (Adm, AM) peptide. This gene is located on human chromosome 11p15.
Immunogen
human MR-Pro ADM recombinant protein.
Application
Anti-MR-pro ADM has been used in immunoblotting (~5 kDa) and indirect enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Mid-regional pro-adrenomedullin (MR-proADM) has been suggested as a biomarker for plasma concentrations of adrenomedullin as it is stoichiometrically generated and relatively stable in the plasma. Upregulated levels of MR-pro ADM were associated with an increased risk of mortality and morbidity in patients with heart failure, independent of natriuretic peptides. It can stimulate vascular endothelial barrier function.
Physical form
a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Current heart failure reports, 9(3), 244-251 (2012-06-27)
Circulating biomarkers have become increasingly important in diagnosing and risk-stratifying patients with heart failure (HF). While the natriuretic peptides have received much focus in the past decade, there is increasing interest in the role of other circulating biomarkers such as
Mid-Regional Pro-Adrenomedullin (MR-proADM) as a Biomarker for Sepsis and Septic Shock: Narrative Review
Elevated levels of adrenomedullin in eutopic endometrium and plasma from women with endometriosis
Matson BC, et al.
Fertility and Sterility, 109(6), 1072-1078 (2018)
Sex-specific effects of NLRP6/AVR and ADM loci on susceptibility to essential hypertension in a Sardinian population
Glorioso N, et al.
PLoS ONE, 8(10), e77562-e77562 (2013)
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