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SAB4200223

Sigma-Aldrich

Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-phospho-ALS10, Anti-phospho-TAR DNA binding protein 43, Anti-phospho-TARDBP, Anti-phospho-TARDP43

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

mouse, human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using HeLa cells
western blot: 1-2 μg/mL using HepG2 and A431 cell lysates

UniProt accession no.

Q13148

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer409)

Gene Information

human ... TARDBP(23435)
mouse ... Tardbp(230908)

General description

Transactive response DNA-binding protein 43 (TDP-43) belongs to the family of heterogeneous nuclear ribonucleoproteins (hnRNPs). It has two RNA-recognition motifs and a glycine-rich C-terminal sequence. TDP-43 is expressed in heart, lung, liver and brain, which is localized to the nucleus..

Immunogen

synthetic peptide containing phosphorylated Ser409 of human TDP-43 conjugated to KLH. The corresponding sequence is identical in mouse TDP-43

Application

Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • immunohistochemistry
  • immunoblotting
  • immunofluorescence

Biochem/physiol Actions

Transactive response DNA-binding protein 43 (TDP-43) binds to single stranded RNA. It regulates transcription regulation in human immunodeficiency virus (HIV). TDP-43 is the major ubiquinated component of cytoplasmic inclusions in frontotemporal lobe degeneration subtype (FTLD-U) and amyotrophic lateral sclerosis (ALS). Pathological TDP-43 forms abnormal inclusions in neuronal perikarya and neurites. Mutations in TDP-43 is associated with ALS. Abnormal phosphorylation of TDP-43 at Ser409/410 is observed in FTLD-U and ALS, leading to apoptosis.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Emily Feneberg et al.
Molecular neurobiology, 55(10), 7789-7801 (2018-02-21)
TDP-43 accumulates in nerve cells of nearly all cases of amyotrophic lateral sclerosis (ALS; the commonest form of motor neuron disease) and in the majority of Tau-negative frontotemporal lobar degeneration (FTLD). There is currently no biochemical test or marker of
Retrotransposon activation contributes to neurodegeneration in a Drosophila TDP-43 model of ALS
Krug L, et al.
PLoS Genetics, 13(3), e1006635-e1006635 (2017)
Yuting Ren et al.
Frontiers in neurology, 12, 663637-663637 (2021-07-02)
Objective: The aim of this study was to measure both plasma and cerebrospinal fluid (CSF) TAR DNA-binding protein 43 (TDP-43) and phosphorylated TDP-43 (pTDP-43) levels in sporadic amyotrophic lateral sclerosis (sALS) patients, and to compare them with that of healthy
TDP-43-mediated neurodegeneration: towards a loss-of-function hypothesis?
Broeck LV, et al.
Trends in Molecular Medicine, 20(2), 66-71 (2014)
Lisa Krug et al.
PLoS genetics, 13(3), e1006635-e1006635 (2017-03-17)
Amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) are two incurable neurodegenerative disorders that exist on a symptomological spectrum and share both genetic underpinnings and pathophysiological hallmarks. Functional abnormality of TAR DNA-binding protein 43 (TDP-43), an aggregation-prone RNA and
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