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Key Documents

MD045004

Sigma-Aldrich

M9 SeMET High-Yield Growth Medium Kit

for the preparation of 50 liters of medium

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About This Item

UNSPSC Code:
12352200

shipped in

dry ice

storage temp.

−20°C

General description

The M9 SeMET (selenomethionine) High-Yield Growth Medium Kit is used for cell culture in IPTG-inducible bacterial expression systems leading to the production of SeMET-labeled proteins for crystallographic studies using MAD. The high-yield growth media kit is designed to simplify the procedures to grow cells and eliminate the need for sterilization and weighing out chemicals, saving time and cost by just adding the components one by one. The M9 SeMET High-Yield Growth Medium Kit has been validated and optimized for structural genomics laboratories to double the cell yield and speed up protein production.

Kit Components Only

Product No.
Description
  • M9 salts (P/N/C) + non-inhibitory amino acid cocktail (Part A) 50 pouches/set
  • Mineral Supplements (Part B) 2 x 250
  • Antibiotics + Vitamins (Part C) 1 ea
  • Inhibitory Amino Acid Cocktail + SeMet (Part D) 4 pouches/set
  • IPTG (Part E) 1 pouch/set

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Carc. 1B - Eye Irrit. 2 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual (2001)

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