The kit is designed to detect total polyamines and is not specific for putrescine, spermidine, or spermine individually. The enzyme that metabolizes polyamines is selective for diamines over monoamines, so it is unlikely that chitosan will interfere significantly, unless it is present at high concentrations. The name of the enzyme is considered proprietary information.
MAK349
Total Polyamine Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
Synonym(s):
Fluorescent Polyamine Quantification Kit
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About This Item
UNSPSC Code:
12161503
Pricing and availability is not currently available.
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usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer; aging/geriatric diseases
storage temp.
−20°C
General description
Polyamines are small organic molecules bearing two or more primary amine moieties. Biogenic polyamines such as spermidine, putrescine and spermine function as transcellular signaling molecules and are involved in diverse biological processes. Although small amounts of polyamines are synthesized in cells, larger quantities are often encountered as a result of putrefaction and decay, as they are the direct product of decarboxylation of amino acids such as methionine, lysine and arginine. The enzyme ornithine decarboxylase generates the polyamine putrescine, which is not only responsible for repulsive odor but is also implicated in cancer. Intracellular polyamines readily bind DNA and are critical to preventing oxidative DNA damage and directing DNA double-strand break repair pathways. Polyamine levels decline with age and dietary supplementation of the polyamine spermidine has recently been shown to reduce age-related oxidative stress and extend lifespan in mouse models of aging.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of polyamine concentrations in tissues (e.g. intestine, lung) and biological fluids (e.g. saliva)
Principle
The Total Polyamine Assay Kit enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 μM in tissue lysates and other samples such as saliva.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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How specific is MAK349 in detecting putrescine, spermidine, and spermine? Can the kit be used for a sample containing chitosan?
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