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MAK184

Sigma-Aldrich

Succinate Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

Succinic Acid Assay Kit

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; immunological diseases

storage temp.

−20°C

General description

Succinate (succinic acid) is a citric acid cycle intermediate and functions in the transport of electrons to the mitochondrial electron transport chain. It also functions in innate immune signaling and induces IL-1β production during inflammation. Succinates have been often used for the treatment of hypertension. Succinate measurements can be useful for studying its metabolic, immunological, and therapeutic activities.

Application

Succinate Colorimetric Assay Kit has been used to measure the concentration of succinate.

Suitability

Suitable for the detection of succinate in a variety of samples such as cell and tissue culture supernatants and in liquid samples such as wine.

Principle

The Succinate Colorimetric Assay Kit is a highly sensitive assay for determining succinate levels (ranging from 2-10 nmole/well) in a variety of samples. Succinate concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the succinate present.

Kit Components Only

Product No.
Description

  • Succinate Assay Buffer

  • Succinate Converter

  • Succinate Enzyme Mix

  • Succinate Substrate Mix

  • Succinate Developer

  • Succinate Standard

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism.
Li J, et al.
Nature Communications, 8(1), 1322-1322 (2017)
Metoprolol succinate extended release/hydrochlorothiazide combination tablets.
Hainer JW and Jennifer S
Vascular Health, 3(3), 279-279 (2007)
Yurong Zhang et al.
Redox biology, 28, 101365-101365 (2019-11-11)
Though succinate accumulation is associated with reactive oxygen species (ROS) production and neuronal injury, which play critical roles in epilepsy, it is unclear whether succinate accumulation contributes to the onset of epilepsy or seizures. We sought to investigate changes in
Moran Fremder et al.
Cell reports, 36(6), 109521-109521 (2021-08-12)
The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology. However, the role that bacterial metabolites play in communicating with host cells during inflammatory diseases is poorly understood. Here, we aim to identify the microbiota-determined output
Sarah L Longnus et al.
Transplantation direct, 8(1), e1265-e1265 (2021-12-23)
Heart transplantation with donation after circulatory death (DCD) has become a real option to increase graft availability. However, given that DCD organs are exposed to the potentially damaging conditions of warm ischemia before procurement, new strategies for graft evaluation are

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