Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

MAK092

Sigma-Aldrich

Peroxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

Peroxidase (EC 1.11.1.7) is an enzyme found broadly in biological systems that utilizes hydrogen peroxide in the oxidation of various substrates.[1] The Peroxidase Activity Assay Kit provides a simple and direct procedure for measuring peroxidase activity in a variety of biological samples.

Application

Peroxidase Activity Assay Kit has been used to quantify the activity of peroxidase.[2][3]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the determination of peroxidase activity in cell culture supernatant, serum, plasma, urine, and other biological fluids.

Principle

Peroxidase catalyzes the reaction between H2O2 and the probe, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the peroxidase activity present. One unit of peroxidase is defined as the amount of enzyme that reduces1.0 μmole of H2O2 per minute at 37°C.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8E25K07720
8H30K07720
8E09K07720
7L11K07720
7D28K07720

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Agnieszka Kiełbowicz-Matuk et al.
Frontiers in plant science, 13, 965098-965098 (2022-09-27)
The transition from vegetative growth to reproductive development is a critical developmental switch in flowering plants to ensure a successful life cycle. However, while the genes controlling flowering are well-known in model plants, they are less well-understood in crops. In
Sujitha Balakrishnan Sulochana et al.
Frontiers in bioengineering and biotechnology, 8, 585632-585632 (2020-11-17)
Scenedesmus quadricauda CASA CC202, a potent freshwater microalga is being used as a biofuel feedstock, which accumulates 2.27 fold lipid during nitrogen stress induction. Upon nitrogen starvation, S. quadricauda undergoes biochemical and metabolic changes that perturb the cell to cope
Prasad Kottayil Padmanabhan et al.
Cell death & disease, 7(10), e2406-e2406 (2016-10-14)
DDX3 is a highly conserved member of ATP-dependent DEAD-box RNA helicases with multiple functions in RNA metabolism and cellular signaling. Here, we describe a novel function for DDX3 in regulating the mitochondrial stress response in the parasitic protozoan Leishmania. We
4.09 - Peroxidases
Charlene A M
Comprehensive Toxicology, 4, 169-184 (2010)
Albrecht Serfling et al.
Frontiers in plant science, 7, 1668-1668 (2016-11-25)
Puccinia triticina f. sp. tritici (Eriks.), the causal agent of leaf rust, causes substantial yield losses in wheat production. In wheat many major leaf rust resistance genes have been overcome by virulent races. In contrast, the prehaustorial resistance (phr) against
View All Related Papers

Protocols

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. The protocol for the kit says this should be used with 96 well plates and a 96-well spectrophotometer. Can this be used for single reactions?

    1 answer

    1. This product has not been tested for single reactions. Sample peroxide values are determined by comparison to the standard curve which is prepared in the same plate. The end user would have to optimize for single reaction use. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/418/201/mak092pis-ms.pdf

      Helpful?

  4. If we are going to use the POD kit to evaluate the activity in broccoli juice do we need to extract and purify the enzyme? What is the lowest detection level

    1 answer

    1. If the enzyme is already in solution (that is, already in the juice), then no further sample preparation would be required. The only issue might be the intense color of the broccoli juice.

      The sample should be diluted sufficiently so the juice does not interfere with the detection (whether colorimetric or fluorometric). Also, if the broccoli juice is not clear, it is recommended to centrifuge samples before testing them. In other words, if there is insoluble particulate matter in the broccoli juice, it may need to be removed to prevent light scattering.

      The lowest detection limit for the kit is 1.0 µmol.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service