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M8065

Sigma-Aldrich

O6-Methylguanine-DNA Methyltransferase human

≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ≥10,000 units/mg protein

Synonym(s):

AGT, MGMT

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About This Item

MDL number:
MFCD03792500
UNSPSC Code:
12352200

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥10,000 units/mg protein

mol wt

~21 kDa
apparent mol wt ~25 kDa by SDS-PAGE

UniProt accession no.

P16455

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MGMT(4255)

Biochem/physiol Actions

O6-Methylguanine-DNA methyl transferase (MGMT), containing 207 amino acids, is a ubiquitous DNA repair enzyme that removes O6-alkyl-guanine, primarily O6-methylguanine, lesions from damaged DNA.
O6-Methylguanine-DNA methyl transferase (MGMT), containing 207 amino acids, is a ubiquitous DNA repair enzyme that removes O6-alkyl-guanine, primarily O6-methylguanine, lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. Its action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein, forming a stable thioether linkage. The cysteine sulfhydryl moiety is not regenerated and therefore, MGMT is frequently classified as a suicide enzyme. Since the reaction irreversibly inactivates the enzyme, the repair capacity for O6-methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents.

Unit Definition

One unit is the amount of protein that removes 50% of the methyl groups of 0.5 pmol double stranded DNA oligonucleotide substrate containing an O6-methylguanine in 10 minutes at 37 °C.

Physical form

Solution in 50% glycerol containing 20 mM Tris, pH 7.5, 1 mM EDTA, 200 mM NaCl, and 1 mM DTT.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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