In this assay, the activity of L-Lactic Dehydrogenase is determined by observing the decrease in absorbance at 340 nm for NADH, rather than the formation of NAD+. The method utilizes a continuous spectrophotometric rate determination to monitor the absorbance decrease at 340 nm, corresponding to NADH absorbance.
L1254
L-Lactic Dehydrogenase from rabbit muscle
Type XI, lyophilized powder, 600-1,200 units/mg protein
Synonym(s):
Anaerobic Lactate Dehydrogenase, Lactate, NAD-lactate dehydrogenase, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
Sign Into View Organizational & Contract Pricing
Select a Size
1000 UNITS
$127.00
5000 UNITS
$209.00
25000 UNITS
$642.00
50000 UNITS
$1,210.00
100000 UNITS
$2,180.00
All Photos(3)
Select a Size
Change View
1000 UNITS
$127.00
5000 UNITS
$209.00
25000 UNITS
$642.00
50000 UNITS
$1,210.00
100000 UNITS
$2,180.00
About This Item
Recommended Products
biological source
rabbit muscle
Quality Level
type
Type XI
form
lyophilized powder
specific activity
600-1,200 units/mg protein
mol wt
140 kDa
composition
protein, 90-100%
storage condition
(Keep container tightly closed in a dry and well-ventilated place)
technique(s)
activity assay: suitable
color
white
foreign activity
pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Research area: Cell Signaling
Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
Application
L-Lactic Dehydrogenase from rabbit muscle has been used:
- as a component of activation and relaxing solution in ATPase activity and isometric steady-state tension measurements with muscle fiber[1]
- in Trypanosoma congolense pyruvate kinase activity assay[2]
- in pyruvate kinase (PK) assay with rice plastidic PK enzyme OsPK2[3]
Biochem/physiol Actions
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Muscle-type Lactic Dehydrogenase (LDH) participates in metabolic pathways and its activity is essential for anaerobic glycolysis. LDH activity is inhibited by ascorbate. LDH regenerates nicotinamide adenine dinucleotide (NAD+) from NADH and is industrially useful in poly(lactic acid) production.[4] In the absence of oxygen, LDH participates in a fermentation reaction, catalyzes pyruvate into lactic acid, and oxidizes nicotinamide adenine dinucleotide (NADH) to NAD+. Therefore, LDH mediates the production of NAD+ essential anaerobic glycolysis pathway. Through this LDH helps maintain the physiological and biochemical functions of the cell in the absence of oxygen.
Unit Definition
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Analysis Note
Protein determined by biuret.
antibody
enzyme
Product No.
Description
Pricing
inhibitor
Product No.
Description
Pricing
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Matthew Warren Eggert et al.
Applied biochemistry and biotechnology, 165(2), 676-686 (2011-06-01)
In order to evaluate the effectiveness of L: -lactate dehydrogenase (LDH) from rabbit muscle as a regenerative catalyst of the biologically important cofactor nicotinamide adenine dinucleotide (NAD), the kinetics over broad concentrations were studied to develop a suitable kinetic rate
Farhana A and Lappin. SL
Biochemistry (2022)
Belén Torrado et al.
Biomedical optics express, 12(7), 3760-3774 (2021-08-31)
We describe a method based on a pair of transmission filters placed in the emission path of a microscope to resolve the emission wavelength of every point in an image. The method can be applied to any type of imaging
John Jeshurun Michael et al.
Journal of the American Heart Association, 5(3), e002777-e002777 (2016-03-24)
We hypothesized that the functional effects of R206L-a rat analog of the dilated cardiomyopathy (DCM) mutation R205L in human cardiac troponin T (TnT)-were differently modulated by myosin heavy chain (MHC) isoforms and T204E, a protein kinase C (PKC) phosphomimic of
Yicong Cai et al.
Plant biotechnology journal, 16(11), 1878-1891 (2018-03-27)
Starch is the main form of energy storage in higher plants. Although several enzymes and regulators of starch biosynthesis have been defined, the complete molecular machinery remains largely unknown. Screening for irregularities in endosperm formation in rice represents valuable prospect
Articles
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
-
In the assay, the UV-Vis absorbance at 340 nm is always showing negative. If lactate continues to form over time, shouldn't the absorbance become more positive?
1 answer-
Helpful?
-
-
What are products that only have the quality release date on the COA and do not have an expiration or retest date?
1 answer-
Certain products are not included in the retest or expiration date programs as there is no indication to suggest that they are unstable. These products will only display the Release Date on the certificate of analysis, with no stated Retest, Expiration, or Use-by Date. It is advised to handle these products according to the defined conditions outlined in our product literature and website product descriptions. It is recommended for end users to regularly inspect these products to ensure they perform as expected. For such products, our standard warranty of 1 year from the date of shipment applies. For more information on our warranty, please refer to the terms and conditions of sale.
Helpful?
-
-
is the substrate given for the enzyme lactate or lactic form, and may I know why it is L-lactic dehydrogenase and not L-lactate dehydrogenase?
1 answer-
The terms tend to be used interchangeably. Lactate is the anion of the conjugate base lactic acid.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/216/153/l1254enz.pdfHelpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
