I9516

2-Propanol

≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent

• Synonym(s): sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
• Linear Formula: (CH₃)₂CHOH
• CAS Number: 67-63-0
• Molecular Weight: 60.10
• Beilstein: 635639
• EC Number: 200-661-7
• MDL number: MFCD00011674
• UNSPSC Code: 12352104
• PubChem Substance ID: 24896158
• NACRES: NA.52

Properties

• product name: 2-Propanol, for molecular biology, BioReagent, ≥99.5%
• grade: for molecular biology
• vapor density: 2.1 (vs air)
• vapor pressure: 33 mmHg ( 20 °C); 44 mmHg ( 25 °C)
• product line: BioReagent
• Assay: ≥99.5%
• form: liquid
• autoignition temp.: 750 °F
• expl. lim.: 2.0-12.7 %, 93 °C
• technique(s): DNA extraction: suitable; RNA extraction: suitable
• impurities: ≤0.2% water; water
• color: colorless
• refractive index: n20/D 1.377 (lit.)
• bp: 82 °C (lit.)
• mp: −89.5 °C (lit.)
• solubility: water: soluble (completely)
• density: 0.785 g/mL at 25 °C (lit.)
• suitability: suitable for precipitation of nucleic acids
• storage temp.: room temp
• SMILES string: CC(C)O
• InChI: 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
• InChI key: KFZMGEQAYNKOFK-UHFFFAOYSA-N

Description

General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

• DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
• Protein crystallization.
• Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.

Application

2-Propanol has been used:

• in DNA and RNA extraction
• for determination of hydroxyproline levels(The Merck Index, 12th ed)
• for checking cell viability by MTT assay
• in microarray hybridization

Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H319,H336
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 53.6 °F - closed cup
• Flash Point(C): 12.0 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves