GE17-0402-01
HiTrap® Protein A High Performance
Cytiva 17-0402-01, pack of 5 × 1 mL
Synonym(s):
Protein A Affinity Column
About This Item
Recommended Products
ligand
native protein A
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-0402-01
storage condition
(20% Ethanol)
parameter
flow rate
<4 mL/min flow rate
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-10
working range
3-9
storage temp.
2-8°C
Related Categories
General description
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
Features and Benefits
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
General instructions for affinity purification using HiTrap columns from Cytiva.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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