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Product Name
MOPC 315, 85022106
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Immunoglobulin A lambda
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse myeloma
Cell Line Description
Established in vitro from the MOPC 315 plasmacytoma induced by mineral oil injections in a BALB/c mouse. Secretes an IgA lambda antibody which recognises DNP-TNP substituted proteins. Cells are killed by low concentrations (10nM) of dexamethasone.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10-20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 1-9x100,000 cells/ml; 5% CO2; 37°C. Do not allow cell density to rise above 1,000,000 cells/ml as rapid death occurs at higher concentrations.
Other Notes
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