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PNV35B

01080924, Reactivity: Anti-Osteoclasts (human foetal), ECV304 and U937 cells

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About This Item

UNSPSC Code:
41106514

ImmunoDon

BALB/c mice

Myeloma

Ag876

Reactivity

Anti-Osteoclasts (human foetal), ECV304 and U937 cells

technique(s)

cell culture | mammalian: suitable

isotype

IgM plus IgG

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Anti-Osteoclastoma Hybridoma

Cell Line Description

135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.

Immunogen

Osteoclastoma tumour

Application

Immunocytochemistry

Subculture Routine

Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO2, 37°C,

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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