C9616

Anti-Cytochrome c antibody produced in sheep

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CYC
• MDL number: MFCD01864291
• UNSPSC Code: 12352203

Properties

• biological source: sheep
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 15 kDa
• species reactivity: rabbit, rat, human, canine
• concentration: ~0.5 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using human heart tissue; indirect immunofluorescence: 5-10 μg/mL using human MCF-7 cells; western blot: 0.1-0.2 μg/mL using whole extracts of MCF−7, Jurkat, Rat−1, MDCK cells and extract of rat kidney or rat heart
• UniProt accession no.: P99999
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CYCS(54205) ; rat ... Cycs(25309)

Description

General description

Anti-Cytochrome c produced in sheep using purified rabbit cytochrome c conjugated to keyhole limpet hemocyanin (KLH).

Immunogen

rabbit cytochrome c.

Application

Anti-Cytochrome c antibody produced in sheep has been used in:

• immunocytochemistry
• immunostaining
• immunoblotting
• immunohistochemistry

Biochem/physiol Actions

Cytochrome c controls cellular electron transport and energy metabolism. It is involved in the transfer of electrons between complex III and complex IV in the mitochondrial electron transport chain. It is involved in apoptosis and activates the death protease caspase-3 (CCP32). The presence of Bcl-2 on the organelles inhibits the release of cytochrome c from mitochondria during apoptosis. Along with Apaf-1, and procaspase-9, it forms an essential component of vertebrate ”apoptosome”. Activation of caspase-9 and other capsases directs apoptosis. Serum cytochrome c is a sensitive apoptotic marker in vivo, and increased serum cytochrome c level can serve as a negative prognostic marker.

Target description

Cytochrome c is an electron transport protein released from mitochondria as an early committed event in apoptosis. Cytochrome c and dATP are cofactors for the mammalian apoptosome, which is composed of Apaf-1, Bcl-2, and procaspase 9. When caspase 9 is activated, activation of other caspases follow including the death protease caspase 3. The release of cytochrome c is inhibited by the presence of Bcl-2 on these organelles preventing the initiation of apoptosis. In cells induced by several apoptotic agents (such as UV irradiation, staurosporine, and overexpression of Bax), caspase inhibitors do not prevent cytochrome c release.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable