to generate ConA blast targets from B6 and lpr spleen cells[2]
to stimulate splenic lymphocytes from estrogen-and placebo-treated mice[3]
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Frontiers in immunology, 12, 683631-683631 (2021-08-10)
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ConA 제품 (C0412)에 관해 질문 드립니다. ConA가 Ca2+, Mn2+ 이온이 존재해야 활성화가 된다고 하는데 해당 제품은 powder형태이며 이는 따로 용액이 필요합니다. 그렇다면 이 제품 안에 저 금속 이온이 함께 들어있는 건지, 아니면 Sigma 제품 중에 이 제품을 혼합할 수 있는 PBS가 따로 있는지(있다면 카탈로그 번호 알려주셨으면 합니다) 궁금합니다.
1 answer
Technical Support
·a year ago
Concanavalin A has 2 metal binding sites per monomer. Calcium and Manganese are required to bind these sites in order for sugar binding to occur. It is recommended to prepare stock solutions in PBS containing Ca2+ and Mn2+.
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