![N-[2-(Dansylamino)ethyl]maleimide BioReagent, suitable for fluorescence, ≥99.0% (HPLC)](/deepweb/assets/sigmaaldrich/product/structures/318/294/efefa674-7883-4fc7-ad57-ea90ff85069a/640/efefa674-7883-4fc7-ad57-ea90ff85069a.png)
The B1267 Biotin-maleimide is a white powder that is soluble in DMSO at 10mg/ml, resulting in a clear colorless solution. A stock solution of the product in DMSO at 20 mg/ml can be prepared without the need for heat. This solution can then be diluted 1:10 with an aqueous buffer system, such as PBS or other. Concentrations of 40-50 mg/ml can also be prepared, but heat, i.e. 40°C, is needed. For most applications, a stock of 20 mg/ml would be suitable.
B1267
Biotin-maleimide
≥95% (TLC), powder
Synonym(s):
N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide
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25 MG
$286.00
100 MG
$907.00
About This Item
Empirical Formula (Hill Notation):
C20H29N5O5S
CAS Number:
Molecular Weight:
451.54
MDL number:
UNSPSC Code:
12352203
PubChem Substance ID:
NACRES:
NA.46
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Quality Level
Assay
≥95% (TLC)
form
powder
solubility
acetic acid: 20 mg/mL
storage temp.
2-8°C
SMILES string
O=C(CCCCCN1C(=O)C=CC1=O)NNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23
InChI
1S/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1
InChI key
VAPZWEFPQRDVHR-NJSLBKSFSA-N
Related Categories
General description
Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups[1].
Application
Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides[2].
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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