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45245

Sigma-Aldrich

Eosin 5-isothiocyanate

≥95.0% (UV)

Synonym(s):

EITC

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About This Item

Empirical Formula (Hill Notation):
C21H7Br4NO5S
CAS Number:
60520-47-0
Molecular Weight:
704.96
MDL number:
MFCD00082502
UNSPSC Code:
12352200
PubChem Substance ID:
57650268

Assay

≥95.0% (UV)

fluorescence

λex 521; λem 544 (pH 9.0)

storage temp.

2-8°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S

InChI

1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)

InChI key

MCXYFYWNOWPDMA-UHFFFAOYSA-N

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Application

Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.

Other Notes

Acceptor fluorophore. Measurement of rotational diffusion of proteins[1]; In fluorescence energy transfer studies[2][3]

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF9397
BCCB8383
BCBZ5677
BCBW2640
BCBV2697

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Yan Fu et al.
Journal of immunological methods, 289(1-2), 57-64 (2004-07-15)
A solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA) for the determination of human complement3 (C3) based on a sandwich type assay and a labelled avidin-biotin (LAB) type assay was described. The anti-human complement3 and avidin were labeled with eosin5-isothiocyanate. On
J W Erickson et al.
Biochemistry, 34(27), 8693-8700 (1995-07-11)
In this work, we have used resonance energy transfer to determine the relative positions of a reactive cysteine residue on the gamma subunit of the retinal cyclic GMP phosphodiesterase (gamma PDE) and a reactive lysine residue on the alpha subunit
A D Horowitz et al.
Biochemistry, 32(37), 9513-9523 (1993-09-21)
The self-association of pulmonary surfactant protein SP-C in lipid vesicles was studied using fluorescence energy transfer. Bovine SP-C was labeled with two fluorescent probes, succinimidyl 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoate and eosin isothiocyanate, on the amino terminus of the protein, producing NBD-SP-C and EITC-SP-C
Photohemolysis of human erythrocytes labeled in band 3 with eosin-isothiocyanate.
J P Pooler et al.
Photochemistry and photobiology, 44(4), 495-499 (1986-10-01)
Ya-lan Wu et al.
Guang pu xue yu guang pu fen xi = Guang pu, 24(12), 1597-1600 (2005-04-15)
In this paper, the medium (pH, organic solvent and surfactant) effects on fluorescence of eosin-5-isothiocyanate (eosin-ITC) have been studied in detail. The results show that the pH and polarity of system studied are two great important influence factors on fluorescence
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