MABF2323

Anti-HLA-A02:01 EBV-EBNA1 Antibody, clone MAB13

• Synonym(s): Epstein-Barr nuclear antigen 1;EBNA-1;EBV nuclear antigen 1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MAB13 (also referenced as E1), monoclonal
• mol wt: calculated mol wt 56.43 kDa
• purified by: using protein G
• species reactivity: human, mouse
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: C-terminal
• Protein ID accession no.: Unknown
• UniProt accession no.: P03211
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... EBNA1(3783774)

Description

General description

Epstein-Barr nuclear antigen 1 (UniProt: P03211; also known as EBNA-1, EBV nuclear antigen 1) is encoded by the EBNA1 (also known as BKRF1) gene (Gene ID: 3783709) in Epstein-Barr virus (EBV). EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. Although the majority of virus infected individuals remain healthy, EBV has been implicated in the pathogenesis of African Burkitt s lymphoma and nasopharyngeal carcinoma. EBNA1, a multi-functional protein, plays an important role in maintaining EBV in the host cell and has been implicated in host cell immortalization. It plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. It is also reported to direct the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes and is known to activate the transcription of several viral latency genes. By counteracting the stabilization of host p53, it also reduces apoptosis and increases host cell survival. EBNA1 is also a risk factor in a variety of malignancies and is the only viral protein found in all EBV-related malignancies. Its C-terminal region contains a dimerization domain and a DNA binding domain (aa 459-607). Although this domain is essential for its replication function of EBNA1, it is not sufficient for this activity and the N-terminal half of EBNA1 is also required.

Specificity

Clone MAB13 (E1) is a mouse monoclonal antibody that detects EBNA1. It targets an epitope within 9 amino acids from the C-terminal region.

Immunogen

Membrane free HLA-A201 with EBV (strain B95-8) EBNA1 (FMVFLQTHI) 562-570 recombinant protein.

Application

Quality Control Testing

Evaluated by Flow Cytometry in T2 cells pulsed with EBNA1 peptide (562-570).

Flow Cytometry Analysis (FC): 1 μg of this antibody detected HLA-A2-EBV EBNA1 in one million T2 cells pulsed with EBNA1 peptide (562-570).

Tested Applications

Immunofluorescence Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Immunofluorescence applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

Immunohistochemistry Applications: A representative lot detected HLA-A02:01 EBV-EBNA1 in Immunohistochemistry applications (Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

Western Blotting Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Western Blotting applications (Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

Flow Cytometry Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Flow Cytometry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Immunocytochemistry Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Immunocytochemistry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Surface plasmon resonance: A representative lot detected HLA-A02:01 EBV-EBNA1 in Surface plasmon resonance applications (Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

ELISA Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in ELISA applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HLA-A02:01 EBV-EBNA1, clone MAB13, Cat. No. MABF2323, is a mouse monoclonal antibody that detects EBNA1 and is used in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Surface Plasmon Resonance & Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable