biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
7E5.14.5, monoclonal
mol wt
calculated mol wt 30.61 kDa
observed mol wt ~18 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
General description
Interleukin-1 alpha (UniProt: P01583; also known as IL-1 alpha, Hematopoietin-1) is encoded by the IL1A (also known as IL1F1) gene (Gene ID: 3552) in human. IL-1a is constitutively expressed in several healthy tissues in a steady state and its expression can be increased in response to a number of stimuli. Absolute amounts of IL-1a vary among cell types, but barrier cells, such as endothelial and epithelial cells, express higher amounts at steady state. IL-1a is synthesized with a propeptide (aa 1-112) and is post-translationally modified by phosphorylation at serine 90, myristoylation on lysine 82, and acetylation on lysine 82. After synthesis, pro-IL-1a is localized into the nucleus, cytosol or lysosomal compartment or is displayed on the outer leaflet of the plasma membrane. Its intracellular localization can change in response to specific stimuli. Pro-IL-1a can also be cleaved by calpain to generate IL-1 -N-terminal domain (NTP) and a mature C-terminal IL-1a form. The C-terminal domain is catalytically active and is capable of signaling via IL-1R. Both IL-1a -NTP and pro-IL-1a contains a functional NLS signal and bind HAX1 to allow translocation into the nucleus. In the nucleus, IL-1a -NTP binds transcription regulation factors and activates expression of proinflammatory cytokines and chemokines independently of IL-1R1 signaling. The biological functions of IL-1a also involve stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. (Ref.: Malik, A., and Kanneganti, TD. (2018). Immunol. Rev. 281(1); 124-137; Di Paolo, NC., and Shayakhmetov, DM (2016). Nat. Commun. 17(8); 906-913).
Specificity
Clone 7E5.14.5 is a mouse monoclonal antibody that detects human IL-1a .
Immunogen
Full-length recombinant human IL-1alpha (without the propeptide).
Application
Quality Control Testing
Evaluated by Western Blotting with human recombinant IL-1a.
Western Blotting Analysis: A 1,000 dilution of this antibody detected human recombinant IL-1a.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1a in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with human recombinant IL-1a.
Western Blotting Analysis: A 1,000 dilution of this antibody detected human recombinant IL-1a.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1a in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-1a, clone 7E5.14.5, Cat. No. MABF2299, is a mouse monoclonal antibody that detects Interluekin-1a and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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