Skip to Content
Merck
All Photos(1)

Documents

GF144

Sigma-Aldrich

Epidermal Growth Factor Protein, Human recombinant

The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.

Synonym(s):

EGF

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

Quality Level

100

Assay

>98% (SDS-PAGE)

specific activity

≥1 x 10^7 U/mg

manufacturer/tradename

Chemicon®

impurities

<0.1 ng/μg Endotoxin (of EGF; 1EU/μg)

input

sample type neural stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)

NCBI accession no.

NM_001963.2

UniProt accession no.

P01133

shipped in

dry ice

Related Categories

General description

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Product Source: E.coli

Application

For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.

Linkage

Replaces: GF001

Physical form

Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.

Storage and Stability

The lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.

Analysis Note

Specific Activity: Human EGF is fully biologically active when compared to standards.The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Lenka Munoz et al.
Cancer biology & therapy, 17(4), 355-363 (2016-01-23)
Inflammation is an important factor promoting the progression of glioblastoma. In the present study we examined the contribution of Ras signaling and TNFα/IL-1β cytokines to the development of the glioblastoma inflammatory microenvironment. Enhanced activation of Ras through de-regulated activation of
GUSBP11 Inhibited The Progression of Triple Negative Breast Cancer via Targeting The miR-579-3p/SPNS2 Axis.
Wu, et al.
Cell Journal, 24, 230-238 (2022)
Hui-Fang Guo et al.
Antioxidants (Basel, Switzerland), 9(2) (2020-02-08)
Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats
Seonhee Kim et al.
Frontiers in microbiology, 12, 730892-730892 (2022-01-01)
Rabies is a zoonotic disease caused by rabies virus (RABV). As rabies advances, patients develop a variety of severe neurological symptoms that inevitably lead to coma and death. Unlike other neurotropic viruses that can induce symptoms of a similar range
Weili Ma et al.
Nanomaterials (Basel, Switzerland), 9(3) (2019-03-21)
Magnetic iron oxide (Magnetite, Fe₃O₄) nanoparticles are widely utilized in magnetic resonance imaging (MRI) and drug delivery applications due to their superparamagnetism. Surface coatings are often employed to change the properties of the magnetite nanoparticles or to modulate their biological
View All Related Papers

Protocols

Human Neural Stem Cells Differentiation with TrueGel3D®

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

Related Content

Stem Cell Testing of PES Membrane Containing Stericup® Quick Release Filters

This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service