biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
mol wt
calculated mol wt 21.86 kDa
observed mol wt ~33 kDa
purified by
affinity chromatography
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
affinity binding assay: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
mutation (Frameshift aa145-150)
Gene Information
human ... Unknown(3008)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in human oligodendroglioma cell line SCC163 stably expressing transgenic H1Efs-FLAG-HA.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H1E frameshift mutant in human oligodendroglioma cell line SCC163 stably expressing transgenic H1Efs-FLAG-HA.
Tested Applications
Immunofluorescence Analysis: A representative lot detected Histone H1E frameshift mutant in mESc and H1Eframeshift Knock-in cells (Courtesy of Prof. C. David Allis Lab at Rockefeller University).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H1E frameshift mutant in Human WBC and mESC′s & various other cell lines (Courtesy of Prof. C. David Allis Lab at Rockefeller University).
Affinity Binding Assay: A representative lot of this antibody bound Histone H1E peptide with a KD of 1.0 x 10-12
in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Storage and Stability
Other Notes
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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