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AB3375

Sigma-Aldrich

Anti-NFκB p65 Antibody, phospho-specific (Ser276)

serum, Chemicon®

Synonym(s):

Anti-Anti-CMCU, Anti-Anti-NFKB3, Anti-Anti-p65

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer276)

Gene Information

human ... NFKB1(4790)

Specificity

NFkappaB p65, phosphoSer276. By Western blot the antibody shows minimal reactivity with non-phosphorylated p65 and by ELISA is shows minimal reactivity with the non-phosphorylated form of the immunogen peptide.

Immunogen

Epitope: phospho-specific (Ser276)
Synthetic peptide from human NFkappaB p65, phosphoSer276.

Application

Anti-NFκB p65 Antibody, phospho-specific (Ser276) is a high quality Rabbit Polyclonal Antibody for the detection of NFκB p65 & has been validated in ELISA & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western blot: 1:1,000. A 65 kDa band is observed against human p65. HeLa cells of TNF inducible KBM-5 cells can be used as a positive control.

ELISA: 1:10,000-1:30,000

Optimal working dilutions must be determined by the end user.

Physical form

Rabbit serum. Liquid containing 0.1% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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