Rambach Agar plates are standard 90mm stackable specially designed plates that help increase safety during handling, transport, and incubation. Rambach agar is a selective media for isolation and differentiation of Salmonella sp. from Proteus sp. and other enteric bacteria. Production of acid from propylene glycol is a novel characteristic of Salmonella sp. and is utilized in these media.
Application
Rambach agar is a selective media for isolation and differentiation of Salmonella sp. from food and animal feed, water, and other materials.
Analysis Note
The nutritive substrates in the RAMBACH Agar enable Enterobacteriaceae to multiply readily. Sodium desoxycholate inhibits the accompanying Gram-positive flora. RAMBACH Agar enables species of Salmonella to be differentiated unambiguously from other bacteria by means of a new procedure, for which a patent application has been submitted. This is made possible by adding propylene glycol to the culture medium. Salmonellae form acid with propylene glycol, so that, in combination with a pH indicator, the colonies have a characteristic red colour. In order to differentiate coliforms from Salmonellae, the medium contains a chromogene indicating the presence of β-galactosidase splitting, a characteristic for coliforms. Coliform microorganisms grow as blue-green or blueviolet colonies. Other Enterobacteriaceae and Gram-negative bacteria, such as Proteus, Pseudomonas, Shigella, S. typhi and S. parathyphi A grow as colourless-yellow colonies.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
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EN ISO 6579-1 standard specifies detection of Salmonella spp. in human consumption, animal feed, and environmental samples.
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