Predicted to cross-react with Mouse and Rat (100% homologous) based on sequence homology.
Recognizes phosphorylated PKCη (Thr655). Not expected to cross-react with other PKC isoforms
Immunogen
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PKCη (Thr655).
Application
Immunoblot Analysis: A 0.50 μg/mL dilution of this lot detected phospho-PKCη (Thr655) in RIPA lysate of PMA treated Jurkat cells.
Research Category Signaling
Research Sub Category Cytoskeletal Signaling
This Anti-phospho-PKCη (Thr655) Antibody is validated for use in WB for the detection of phospho-PKCη (Thr655).
Quality
Routinely evaluated by immunoblot.
Target description
~80 kDa
Physical form
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide).
Affinity purified
Storage and Stability
Stable for 2 years at -80°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A novel function for the binder of Arl two (BART) molecule in pancreatic cancer cells is reported. BART inhibits invasiveness of pancreatic cancer cells through binding to a Ca(2+)-dependent, phosphorylated, guanosine triphosphatase (GTPase) membrane fusion protein, annexin7 (ANX7). A tumor
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Protein kinase C (PKC) is a family of at least 10 isozymes involved in the activation of different signal transduction pathways. The exact function of these isozymes is not known at present. Isozyme-selective inhibitors would be important to explain the
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