07-408
Anti-Smad2/3 Antibody
Upstate®, from rabbit
Synonym(s):
MAD, mothers against decapentaplegic homolog 2 (Drosophila), Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD, mothers against DPP homolog 2, SMA
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat
packaging
antibody small pack of 25 μL
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SMAD2(4087)
General description
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFβ family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFbeta′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Specificity
Recognizes human Smad2 Mr 55-60 kDa and Smad3 Mr 50 kDa as determined by using Smad2 and Smad3 transfected Cos cells.
Immunogen
Fusion protein corresponding to amino acids 186-273 of human Smad2.
Application
Anti-Smad2/3 Antibody is a Rabbit Polyclonal Antibody for detection of Smad2/3 also known as MAD-mothers against decapentaplegic-homolog 2 & has been validated in WB.
Immunoblot Analysis: 1:1000-1:2000 dilution detected Smad2/3 in RIPA lysates of HepG2 cells.
Optimal working dilutiuons must be determined by end user.
Optimal working dilutiuons must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Routinely evaluated by Western Blot on Hela lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SMAD2/3 on 10 μg of Hela lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SMAD2/3 on 10 μg of Hela lysates.
Target description
50-60 kDa
Linkage
Replaces: 04-1029
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
HepG2 cell lysate.
HepG2 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
SMAD3 and EGR1 physically and functionally interact in promoter-specific fashion.
Jerome Fortin,Daniel J Bernard
Cellular Signalling null
TGFbeta signaling: receptors, transducers, and Mad proteins.
Massague, J
Cell, 85, 947-950 (1996)
Embryonic overexpression of Galgt2 inhibits skeletal muscle growth via activation of myostatin signaling.
Kumaran Chandraskeharan,Paul T Martin
Muscle and Nerve null
Jennifer Skirkanich et al.
Developmental biology, 357(2), 478-491 (2011-07-12)
Most zygotic genes remain transcriptionally silent in Drosophila, Xenopus, and zebrafish embryos through multiple mitotic divisions until the midblastula transition (MBT). Several genes have been identified in each of these organisms that are transcribed before the MBT, but whether precocious
Expression of transforming growth factor-beta (TGF-beta) in chronic idiopathic cough.
Xie, S; Macedo, P; Hew, M; Nassenstein, C; Lee, KY; Chung, KF
Respiratory Research null
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