XNAB2
Extract-N-Amp™ Blood PCR Kit
sufficient for 100 extractions, sufficient for 100 amplifications
Synonym(s):
Blood direct PCR kit
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About This Item
UNSPSC Code:
41106303
NACRES:
NA.55
Recommended Products
usage
sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions
Quality Level
feature
dNTPs included
hotstart
technique(s)
PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
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General description
The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.
Application
Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).
Features and Benefits
- Efficient 8 minute prep allows greater speed and throughput
- No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
- Simple, 3 step procedure with no special equipment required
- Hot start antibody included for highly specific PCR amplification of genomic DNA
- Compatible with multiple format (single tube or 96-well plate)
- Can be used with whole blood or blood cards
- Extract stable at 4 °C for at least 6 months
Principle
Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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