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S5313

Sigma-Aldrich

Anti-Sir2 (AS-16) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SIR2, Anti-SIR2L1, Anti-SIR2alpha

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~110 kDa

species reactivity

mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:50 using mouse NIH3T3 cells
microarray: suitable
western blot: 1:2,000 using mouse NIH/3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Sirt1(93759)

General description

Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity.

Specificity

Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.

Application

Anti-Sir2 (AS-16) antibody produced in rabbit has been used in
  • western blotting
  • immunoprecipitation
  • antibody microarray

Biochem/physiol Actions

Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). Sir2 protein is an NAD-dependent histone deacetylase. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Progressive loss of SIRT1 with cell cycle withdrawal
Sasaki T, et al.
Aging Cell, 5(5), 413-422 (2006)
Vladimir Ljubicic et al.
Aging, 1(9), 818-830 (2010-02-17)
Our intent was to investigate the mechanisms driving the adaptive potential of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria in young (6 mo) and senescent (36 mo) animals in response to a potent stimulus for organelle biogenesis. We employed chronic electrical
Induction of hypothalamic Sirt1 leads to cessation of feeding via agouti-related peptide
Sasaki T, et al.
Endocrinology, 151(6), 2556-2566 (2010)
Sir2 links chromatin silencing, metabolism, and aging
Guarente L
Genes & Development, 14(9), 1021-1026 (2000)
Phosphorylation regulates SIRT1 function
Sasaki T, et al.
Testing, 3(12), e4020-e4020 (2008)

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