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R6504

Sigma-Aldrich

RPMI-1640 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.

Application

RPMI-1640 Medium is used for the following applications:
  • used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)[1]
  • used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)[2]
  • used for cell isolation and culture[3][4]
  • used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay[5]
  • used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays[6]
  • used for the preparation of antifungal agents[7][8]

Quantity

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000415982
0000415982
0000382657
0000364421
0000354720

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Sook Yee Boo et al.
Scientific reports, 10(1), 18348-18348 (2020-10-29)
The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B lymphocytes and activate macrophage and T lymphocytes, but there are limited
Hui Tian et al.
Analytical chemistry, 88(23), 11384-11389 (2016-11-02)
MicroRNA (miRNA) analysis in a single cell is extremely important because it allows deep understanding of the exact correlation between the miRNAs and cell functions. Herein, we wish to report a highly sensitive and precisely quantitative assay for miRNA detection
M E Klepser et al.
Antimicrobial agents and chemotherapy, 41(6), 1392-1395 (1997-06-01)
Time-kill curves were determined for three isolates of Candida albicans tested against fluconazole and amphotericin B at multiples of the MIC. Fluconazole produced fungistatic activity, with concentration-related growth effects observed over a narrow range of concentrations. Amphotericin B exhibited fungicidal
Manuel Cuenca-Estrella et al.
Antimicrobial agents and chemotherapy, 46(11), 3644-3647 (2002-10-18)
A two-laboratory study was performed to evaluate the correlation between the NCCLS M27-A and EUCAST microdilution procedures for antifungal testing of Candida spp. A panel of 109 bloodstream isolates was tested against amphotericin B, flucytosine, fluconazole, and itraconazole. Overall, the
Mathias Lichterfeld et al.
The Journal of experimental medicine, 200(6), 701-712 (2004-09-24)
Virus-specific CD8(+) T cells are associated with declining viremia in acute human immunodeficiency virus (HIV)1 infection, but do not correlate with control of viremia in chronic infection, suggesting a progressive functional defect not measured by interferon gamma assays presently used.
View All Related Papers

Questions

1–4 of 4 Questions  

  1. how much media is added to prepare 10ml rpmi media?

    1 answer

    1. This product is a powder form of RPMI. This formulation requires 10.4 grams to prepare one liter of medium. The addition of 2.0 g/L sodium bicarbonate is also required. To prepare 10 ml of liquid medium from this item would require 104 mg of the powder product plus the addition of 20 mg of sodium bicarbonate. Note that R8758, the prepared liquid medium of this version of RPMI, may be of interest.

      Please see the link below to compare the powder and liquid medium formulations:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/209/459/r6504for.pdf

      Helpful?

  2. Why doesn't the powder dissolve in deionised water for 3 days?

    1 answer

    1. The product issue you are reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

  3. Buongiorno Gentilissima/o, volevo chiedervi maggiori informazioni per quanto riguarda la differenza tra il terreno R6504-10L e il R4130-10L: sono entrambi in polvere? da cosa deriva la differenza di prezzo? funziona per la crescita di muffe? Grazie

    1 answer

    1. The only difference between these two formulations is the addition of HEPES buffer to the R4103. All other components are identical. None of the classical mammalian culture media are tested for culture or expansion of molds. However, sterile solutions of this media, if handled improperly, have been known to become easily and heavily contaminated the airborne contaminants such as mold spores. To compare these media formulations please see the link below to access the RPMI-1640 formulations table:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/209/459/r6504for.pdf

      Helpful?

  4. is this product available in powder form

    1 answer

    1. Product R6504 is a powdered formulation of RPMI. It is formulated to contain 10.4 grams of powder per liter of medium and requires supplement with 2.0 g/L sodium bicarbonate. Its 1X liquid product equivalent is Product R8758 (https://www.sigmaaldrich.com/US/en/product/sigma/r8758

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