P5280

Sodium pyruvate

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

• Synonym(s): α-Ketopropionic acid sodium salt, 2-Oxopropanoic acid sodium salt, Pyruvic acid sodium salt
• Linear Formula: CH₃COCOONa
• CAS Number: 113-24-6
• Molecular Weight: 110.04
• Beilstein: 3568341
• EC Number: 204-024-4
• MDL number: MFCD00002586
• UNSPSC Code: 12352207
• PubChem Substance ID: 24898615
• NACRES: NA.71

Properties

• product line: BioReagent
• Assay: ≥99%
• form: powder
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• mp: >300 °C (lit.)
• solubility: H₂O: 100 mg/mL
• density: 1.78 at 20 °C
• application(s): metabolomics
• storage temp.: 2-8°C
• SMILES string: [Na+].CC(=O)C([O-])=O
• InChI: 1S/C3H4O3.Na/c1-2(4)3(5)6;/h1H3,(H,5,6);/q;+1/p-1
• InChI key: DAEPDZWVDSPTHF-UHFFFAOYSA-M

Description

General description

Sodium pyruvate, derived from pyruvic acid, finds extensive use in cell biology, cell culture, and biochemical research. As the end-product of the glycolysis pathway, where glucose is converted into pyruvate with the energetic production of ATP (Adenosine Triphosphate), pyruvate serves as an intermediary in numerous metabolic pathways, including sugar metabolism. While cells naturally synthesize sodium pyruvate as an intermediate metabolite in glycolysis, it isn′t universally necessary as a supplement for all cell cultures. However, if cells have been cultivated in a medium enriched with sodium pyruvate, it′s advisable to continue its use as cell growth may diminish without it.Sodium pyruvate is frequently incorporated into cell culture formulations to fortify broths and media, enhancing cell survival by providing an easily-accessible carbohydrate energy source alongside glucose. It′s often added to the growth medium, particularly for rapidly proliferating cells like tumor cells. Furthermore, it participates in amino acid metabolism and initiates the Krebs cycle. Cells can readily utilize sodium pyruvate as a carbon source for energy production and crucial metabolic pathways, eliminating the need for biosynthetic production from glucose or amino acids.Certain cell lines require supplemental pyruvate in the culture media due to their inability to convert glucose or amino acids into pyruvate. Additionally, sodium pyruvate serves as a free radical scavenger, effectively quenching reactive oxygen species (ROS) and contributing to cellular health and viability.

Application

Sodium pyruvate has been used:

• to culture MCF7 ρ0 (human breast cancer cell line) cells as parent cells
• as a supplement in extracellular flux (XF) assay media to incubate cells for measuring oxygen consumption rate (OCR)
• as a supplement in minimum essential medium Eagle to maintain breast cancer (MCF-7) cells
• as a supplement in Dulbecco′s modified Eagle medium (DMEM)-based medium and modified Eagle medium (MEM)-α for testis cell culture to evaluate the effect of medium composition on in vitro SSC maintenance

Biochem/physiol Actions

Sodium Pyruvate has shown antioxidant properties and protective effects against oxygen radicals. Pyruvate is produced as part of glycolysis and is an intermediate in many metabolic pathways. It can be converted into acetyl CoA and enter the TCA Cycle.

Sodium pyruvate can be used as a component in culture broth and media. It exhibits protective effects in various animal models of ischemia-reperfusion injury, hemorrhagic shock, and septic shock when given exogenously.

Features and Benefits

• Cell culture and insect cell culture tested

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioReagent Grade Sodium succinate for your Cell Biology and Biochemical research
• Tested for Mammalian and Insect cell culture applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319
• Precautionary Statements: P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1B
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves