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N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

Synonym(s):

Neutralizing Buffer for Blotting Techniques

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

powder

technique(s)

Southern blotting: suitable

foreign activity

DNase, RNase, none detected

storage temp.

room temp

General description

Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

Application

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

Components

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

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